|DNA base damage and chromatin integrity||Assoc. Prof. Barbara von Loon | NTNU, Trondheim, Norway|
In both normal and cancer cells numerous lethal and mutagenic DNA lesions are formed upon exposure to endogenous and exogenous damaging agents with about 20’000 lesions per cell per day, majority of which represent DNA base damages. An increase in the amount of DNA base damages has been tightly associated with aging, cancer development, as well as onset of different neurological diseases. To maintain the integrity of genetic information organized within chromatin, cells need to repair the DNA or bypass the damage by engaging specialized DNA polymerases.
The aim of our research is to identify different cellular factors that ensure efficient response to DNA damaging agents, in particular the one inducing DNA base damage. Our research focuses on exploring the importance of DNA repair proteins and specialized DNA polymerases in onset and progression of different diseases in particular developmental disorders. By using multidisciplinary approach combining various experimental techniques, including biochemistry, cell biology, genomics, proteomics, 3D cultures, imaging, shRNA/CRISPR libraries, and computational biology we aim to identify pathomechanisms that contribute to developmental disorders and could potentially serve as basis for evolution of novel therapies.